miRNA ISOLATION AND EXPRESSION TRAINING PROGRAM
miRNAs regulate various metabolic and cellular pathways that specifically control cell proliferation, migration, differentiation and signal transduction by negatively affecting the expression of target mRNAs. Numerous microRNAs have been identified that play a role in the transcription and translation of tumor suppressor genes or oncogenes in many solid tumors and hematologic malignancies. The relationship between microRNA expression levels and metastasis and survival in cancer patients was investigated. Since microRNAs have tissue-specific expression profiles and effective and reliable methods of expression quantification can be performed, microRNA-based markers are important in cancer diagnosis, prognosis and follow-up of treatment. In addition, microRNAs are involved in the regulation of radiotherapy and chemotherapy responses of tissues.
To gain knowledge and ability to detect and evaluate the amount of miRNA expression from different samples. The theoretical part of the course will provide information on miRNA isolation methods and details, methods for determining the amount of miRNA, amplification methods and how to normalize them. For the isolation of miRNA, the methods of isolation from classical tissue or cell culture as well as direct isolation from plasma will be discussed and their effectiveness will be examined. Sources of errors and strategies to avoid mistakes will be explained. In the application part, suitable samples prepared for miRNA isolation will be used and individual study will be provided. The quantification and expression of miRNAs obtained will be discussed with visual materials prepared by real-time PCR method. Information will be given about normalization. Clinical evaluation of identified up-regulated and down-regulated miRNA profiles will be performed.
To be able to create a different view of these molecules with the recent developments in the field of research of miRNAs that attract attention with biomarker studies.
At the end of this course participants will:
1. explain and apply miRNA isolation methods from plasma and different tissues
2. be able to quantify miRNA
3. identify miRNA expression profiles
4. apply normalization, basic clinical evaluation issues
Researchers and postgraduate students and specialists in biochemistry.
Necessary knowledge and skills
Molecular genomic techniques should have a basic level of knowledge.
09:00-09:15 Opening Speech: Summarizing the daily schedule
09:15-09:30 Preliminary Test09:30-09:50 Principles of miRNA isolation method
09:50-10:10 miRNA expression
10:10-10:30 Normalization and evaluation
10:30-12:30 miRNA Isolation / quantitation / application (practical course) 12:30-13:30 Lunch
13:30-15:30 miRNA isolation / quantitation /application (practical course)
15:30-16:00 miRNA expression (video demonstration)
16:00-16:20 Normalization and evaluation
16:20-17:00 Q & A, Final test / Discussion and closing
Papillon Zeugma Convention Centre, Belek Antalya - TURKEY
October 27-31, 2019